ProPrime Taq DNA Polymerase

Product Descriptions

ProPrime Taq DNA Polymerase is an engineered variant of Taq DNA Polymerase that offers superior tolerance to endogenous and exogenous inhibitors in clinical specimens featuring fast amplification speed and high sensitivity, making it ideal for rapid PCR and for detection of low abundance target DNA. ProPrime Taq DNA Polymerase is suitable for PCR and dye- or probe-based quantitative PCR (qPCR).

Specifications & Components

Applications

• Direct qPCR/PCR: amplification directly from whole blood, crude fecal samples, and plant materials with no DNA extraction needed.

• Hot start fast qPCR/PCR and ARMS-PCR (Amplification Refractory Mutation System-PCR).

Unit Definition

One unit refers to the amount of enzyme required to catalyze 10 nmol dNTP added into acid-insoluble precipitate at 74°C for 30 min using activated salmon sperm DNA as a template / primer.

Highlights of ProPrime Taq

• Extraordinary Inhibitor Resistance: Engineered for strong resistance to blood and various inhibitors, delivering consistent PCR and qPCR results.

• Exceptionally-Fast Amplification: Achieves rapid amplification speed of 7.5s/kb, significantly reducing experimental time.

• Superior Sensitivity: Offers exceptional sensitivity and reproducibility, enabling precise qPCR results.

• Proven Quality: Adheres to stringent quality standards, providing excellent lot-to-lot consistency.

Key Performance Data

Extraordinary Blood Resistance

Fapon ProPrime Taq DNA Polymerase demonstrates exceptional tolerance to whole blood, with the ability to handle up to 20% whole blood in PCR systems and 10% in qPCR systems. A human endogenous gene was used as qPCR/PCR target.

Broad-spectrum and Potent Resistance to Inhibitors in PCR

*The √ symbol denotes data that exhibited a distinct electrophoretic band.

PCR tests were performed in the presence of a wide range of inhibitors, including impurities from blood, fecal, and soil samples, extraction reagent residues, and PCR enhancers. The results demonstrated that ProPrime Taq DNA Polymerase exhibits robust resistance.

Ultra-Fast Amplification Speed

• Target: Human genomic DNA -2kb

• Extension time: 15sec

• Amplification speed: 7.5sec/kb

• The extension speed of ProPrime Taq DNA Polymerase reaches up to 7.5sec/kb, with high specificity in amplification, superior to the competing products tested.

High Sensitivity

ProPrime Taq DNA Polymerase can amplify as low as 10^-7 ng λ DNA substrate with more distinguished DNA bands which indicates higher sensitivity.

Exceptional Amplification Efficiency, Reproducibility and qPCR Performance

ProPrime Taq DNA Polymerase shows superior performance in terms of amplification efficiency, linearity, and sensitivity when evaluated with plasmid or human genomic DNA samples.