PreciAmp Bst DNA Polymerase

Product Descriptions

• PreciAmp Bst DNA Polymerase is an engineered recombinant DNA polymerase designed for isothermal amplifications. It has an enhanced DNA polymerase and strand-displacement activity over conventional Bst Polymerase, Large Fragment, and lacks 5'→3' and 3'→5' exonuclease activity. 

• PreciAmp Bst DNA Polymerase is highly resistant to high salt environment and complex inhibitors.

• PreciAmp Bst DNA Polymerase is provided with an optimized 10x Reaction Buffer for LAMP and RT-LAMP amplification.

Specifications & Components

Available in 50% glycerol or glycerol-free formats 

Applications

• LAMP or RT-LAMP under optimal conditions.

• Suitable for other applications that rely on Bst DNA Polymerase, Large Fragment.

Unit Definition

One unit is defined as the amount of enzyme that incorporates 25 nmol dNTPs into DNA at 65°C in 30 minutes.

Highlights of PreciAmp Bst DNA Polymerase

• Fast LAMP Amplification and low NTC: Achieves rapid amplification speed, significantly reducing experimental time.

• Improved Sensitivity: Offers exceptional sensitivity and specificity, enabling precise LAMP results.

• Inhibitor Tolerance: Delivers unparalleled resistance to salt, blood and a broad-range of inhibitors, ensuring consistent LAMP performance.

• Primer Compatibility: Compatible with a wide-selection of  DNA/RNA primer sets.

• Outstanding Stability: Adheres to stringent quality standards, providing excellent lot-to-lot consistency.

Key Performance Data

Rapid Speed and High Specificity

(A) Comparing PreciAmp Bst DNA Polymerase to three commercially available Bst DNA polymerases in LAMP assays  with 0.1ng Lambda DNA as template. PreciAmp Bst DNA polymerase is the fastest with the shortest time to result (TTR). (B) PreciAmp Bst DNA Polymerase showed high specificity with no amplification signal in no template control (NTC) reactions within 30min in all 24 repeats.

Superior Sensitivity

(A) Comparing PreciAmp Bst DNA Polymerase to three commercially available Bst DNA polymerases in LAMP assay with ~28 copies of human genomic DNA (targeting the BRCA gene) in 24 repeats. PreciAmp Bst DNA Polymerase exhibited the highest detection rate with fastest detection time. (B) PreciAmp Bst DNA Polymerase in an RT-LAMP assay detected 50 copies of SARS-CoV-2 RNA with 100% positive detection rate in 24 repeats within an average of 6.3 min TTR which highlights the fast speed, high sensitivity and consistency of PreciAmp Bst Polymerase.

Exceptional Tolerance to Salt, Heat and dUTP

 (A) PreciAmp Bst DNA Polymerase is more tolerant to high salt buffe in LAMP assays. (B) PreciAmp Bst DNA Polymerase is more tolerant to heat in LAMP assays. (C) PreciAmp can efficiently incorporate dUTP in LAMP assays where 100% dTTP can be replaced by dUTP.

Exceptional Resistance to Inhibitors

Comparing the inhibitor resistance properties of PreciAmp Bst DNA Polymerase to various commercially available Bst DNA polymerases.  The results show that PreciAmp Bst DNA Polymerase outperforms other products in almost all tested inhibitors.

Compatible with a Wide-selection of Primer Sets

PreciAmp Bst DNA Polymerase is capable to perform robust LAMP amplification with a wide-selection of primer sets and targets. DNA targets: LambdaA and LambdaB primers for Lambda DNA, CFTR and BRCA primers for human DNA.  RNA targets:  ActinB and HMBS primers for human mRNA, N2 and E1 primers for SARS-CoV-2 RNA. TTR (Time To Result) for these targets are all within 8 minutes. No NTC amplification signal were observed for a 30-min incubation with these primers.

Outstanding Stability

No significant change was observed in the LAMP amplification by PreciAmp Bst DNA Polymerase (with Glycerol) after incubation (A) at 37°C for 14 days, (B) at 50℃ for 3 days, or (C) after 30 freeze-thaw cycles.

Outstanding Stability-PreciAmp Bst DNA Polymerase (Glycerol-free)

No significant change was oberserved in the LAMP assay by PreciAmp Bst DNA Polymerase (Glycerol-free) after incubation (A) at 37°C for 14 days, (B) at 50℃ for 4 days, or (C) after 40 freeze-thaw cycles.