MD014 Uracil-DNA Glycosylase
An E. Coli Uracil-DNA Glycosylase (UDG) that removes uracil from uracil-containing DNA.
Product Descriptions
Uracil-DNA Glycosylase (UDG) catalyzes the hydrolysis of the N-glycosidic bond between uracil and sugar groups in uracil-containing single or double-stranded DNA, leaving an apyrimidinic site (AP) in the DNA strand. This enzyme shows no activity on RNA or uracil-free DNA. UDG is widely used to prevent carry-over contamination in PCR reaction. This recombinant E. coli UDG is expressed in and purified from an E. coli strain that carries the cloned E. coli UDG gene. This UDG can be heat inactivated by incubation at 95℃ for 10 minutes.
Applications
Control of PCR carry-over contamination.
Site-specific mutagenesis.
PCR product cloning.
Unit Definition
One unit (U) is defined as the amount of enzyme required to free 1 nmol of uracil from uracil-containing DNA in a reaction containing 70 mM Tris-HCl, pH 7.5 (25°C), 10 mM NaCl, 1 mM EDTA, and100 μg/ml BSA at 37°C for 1 hour.
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