Proteinase K (lyophilized)

Product Descriptions

Proteinase K a highly active broad-range serine protease and is widely used for proteins/enzymes degradation in the preparation of biological specimens. Proteinase K cleaves peptide bond on the carboxylic acid side of aliphatic, aromatic or hydrophobic amino acids residues. The recombmbinant proteinase K is expressed in yeast. Proteinase K remains active under a variety of conditions including pH between 4.0 to 12.0, the presence of 2% SDS or 4M urea. In addition, the activity of proteinase K is not affected by chelating agents such as EDTA.

Specifications & Components

Applications

• Isolation of DNA/RNA.

• Inactivation of RNase, DNase or enzymes in reactions.

• PCR purification.

Unit Definition

One unit (U) is defined as the amount of enzyme needed to yield 1 μg L-tyrosine produced by hydrolysis of casein in 1 min at 55°C, pH 8.0.

Highlights

• Lyophilized powder: ambient temperature shipping and storage and ideal for bulk industrial applications.

• Ultra-long shelf life and stability: Long-term storage at -20°C for 36 months and retains ≥80% activity after 36 months at 25°C.

Key Performance Data

Superior stability and minimal batch to batch variations

Three batches of proteinase K were incubated at -20℃ for 36 months and more than 90% of activity retained after the incubation for each batch of proteinase K.

Three batches of proteinase K were incubated at 25℃ for 36 months and more than 80% of activity retained after the incubation for each batch of proteinase K.